Course Title

Biotechnology II

Course Description

This course is an advanced continuation of Biotechnology I. It will provide the student with practice in in vitro DNA synthesis reactions, programming and use of thermal cyclers for PCR reactions, utilization of real time PCR, Southern and Western blotting techniques, protein extraction and analysis, ELISA technology, and maintenance of animal cell lines.

Contact

Robin Schott
405-743-5554
rscho@okcareertech.org
or
Tina Fugate
405-743-5139
tfuga@okcareertech.org

Knowledge and Skills

In vitro DNA Synthesis Reactions

Programming & Use of Thermal Cyclers for PCR reactions

Utilization of real time PCR

Southern & Western Blotting Techniques

Protein Extraction & Analysis

ELISA technology

Maintenance of animal cell lines

Advanced Bio-Informatics

Diagnostic Biotechnology: Forensics, RFLP DNA fingerprinting, disease diagnosis

Biofuels

Bioremediation

Pharmaceutical Production

Gene Therapy

Human Genome Project

Accountability Measures, Assessments, or Evaluation Procedures

Referenced Standards

 National Science Standards (5^th ed). (1998). National Research Council, Washington, D.C., National Academy of Sciences

Oklahoma Priority Academic Student Skills (2003). Oklahoma State Department of Education-PASS-{ www.sde.state.ok.us}

Industry Alignments

Biotechnology Industry Standards

Curriculum Resources

Clark & Russell, (2000), Molecular Biology Made Simple and Fun, Cache River Press.

Barnum, Susan R., (2007), Biotechnology: An Introduction, Brooks/Cole.

Daugherty, Ellyn, (2007), Biotechnology: Science for the New Millennium, Paradigm Publishing.

Thieman and Palladino, (2004), Introduction to Biotechnology, Pearson Benjamin Cummings.

Micklos and Freyer, (2003), DNA Science: A First Course, Cold Spring Harbor Press.

Alcamo, (2001), DNA Technology: The Awesome Skill, Harcourt Academic Press.

Campbell and Reece, (2005), Biology: AP Edition, Pearson Benjamin Cummings.

This Course Found In These Career Majors