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Health Careers Education
Standard Laboratory Operating Procedures
This course provides the student with a review of the use of common laboratory glassware and utensils, balances, and reading volume. Students are trained in the proper utilization of pipettes and micropipettes, utilization of various heat sources and stirring devices, safe operation of the centrifuge and autoclave, and proper use and care of common laboratory equipment such the microscope and pH meter. Student will also gain practice in preparing chemical solutions from powders and liquids, housekeeping duties, and application of the scientific method. This course emphasizes the need for quality control, consistency, and accuracy in all laboratory procedures.
Lara Skaggs
Disposal of Biological and Chemical Waste

-Demonstrate the correct and effective use of autoclaves when processing biological waste materials.
-Demonstrate the ability to safely neutralize acids and bases prior to disposal.
-Consult MSDS sheets, the facility's chemical hygiene plan, or the laboratory supervisor prior to isposing of any chemicals.
-Demonstrate the ability to safely dispose of chemical waste according to laboratory protocols.

Mass and Volume Measurements

-Review the sensitivity and care of equipment and glassware.
-Demonstrate proper use of pipettes and pipette pumps.
-Select the micropipette required to accurately deliver a given volume by selecting the instrument with the correct volume range.
-Demonstrate how to select, set, and utilize a variety of micropipettes within their designated ranges to accurately measure small volumes.
-Report all mass and volume measurements based upon the precision of the equipment or glassware being used.

Identify and Use Basic Laboratory Equipment

-Review the common equipment used in the laboratory.
-Determine which equipment is appropriate to use for a given task and what units of measurement are used.
-Demonstrate proper use, care and maintenance of all laboratory equipment per laboratory SOPs and/or manufacturer's specifications.
-Properly and safely use and monitor a variety of scientific equipment, including laboratory hoods, centrifuges, incubators, pH meters, microscopes, spectrophotometers, autoclaves, various mixers and
stirrers, water purification systems, water baths, etc.

Solutions, Dilutions, and Buffers

-Identify various expressions for units of concentration.
-Calculate percent and/or ppm concentration of solutions.
-Indicate the correct equation needed to calculate the amount of solute required to make a solution of a specified concentration.
-Accurately calculate the amount of solute required to make various mass/volume, percent mass/volume, and molar solutions.
-Produce solutions of specified concentration and volume through correct and accurate use of the appropriate laboratory equipment.
-Communicate the concept of a serial dilution by using mathematical calculations and diagrams to illustrate the process.
-Perform a dilution series and accurately calculate the end concentration of each solution in the series.
-Discuss the difference between acids, bases, and neutral solutions.
-Define the function of a buffer and give examples of buffers used in a biotechnology lab
-Make several buffers at various volumes, concentrations, and pH.
-Adjust a solution to a specified pH level with the addition of acids or bases as needed.
-Maintain reagent integrity by aliquoting reagents into manageable quantities, storing them properly, avoiding cross-contamination, and using them at the appropriate temperature.
-Sterilize reagents, buffers, media, and solutions where appropriate.

Separation Techniques

-Explain how centrifugation works and identify key safety and sample preparation concerns related to centrifugation.
-Correctly set up and demonstrate the use of simple centrifugation to separate a sample into its component parts.
-Compare the various methods of performing separations using chromatography and identify their applications in laboratory and manufacturing environments.
-List the key steps in the chromatography process and explain the procedure for calculating and applying Rf values in the identification of chemical compounds.
-Calculate and apply Rf values in the identification of chemical compounds.
-Correctly set up and demonstrate the use of simple filtration to separate a sample into component parts.
-Correctly set up and demonstrate the use of simple evaporation to separate a sample into component parts.


-Elucidate the relationship between wavelength and the color of light.
-Cite the colors of different wavelengths of light.
-Describe how a spectrophotometer operates and compare and contrast the characteristics and uses of ultraviolet and visible (white light) spectrophotometers.
-Evaluate the relationship between absorbance and transmittance in spectrophotometry and interpret the meaning of absorbance measurements
-Identify the common parts found on visible spectrophotometers and describe their function.
-Outline the steps of using a visible spectrophotometer.
-Demonstrate how to properly use a spectrophotometer to determine the absorption spectra and lambdamax for colored solutions.
-Utilize a spectrophotometer or colorimeter to build a standard curve for a given set of solutions.
-Determine unknown sample results from using a standard curve and the equation for a line.

Housekeeping and Laboratory Maintenance

-List and describe the basic housekeeping items necessary to assure a contamination-free lab environment.
-Describe the appropriate appearance of the laboratory and status of equipment before exiting each day.
-Demonstrate proper dish washing technique for chemical glassware.
-Explain how failing to maintain a clean and functional working environment with properly cleaned and maintained equipment and
glassware directly relates to quality control concerns and the validity of laboratory findings.
-Describe the disposal of laboratory clean-up materials and tools.
-Schedule, perform, and supervise daily and weekly cleaning duties as assigned.
-Monitor and stock all laboratory supplies as needed.

Quality Assurance & Quality Control

-Set-up and maintain a legal scientific notebook that includes an account of all laboratory procedures, data, and reflections.
-Follow written protocols and oral directions to perform a variety of laboratory and technical tasks.
-Perform specimen collection, label samples, and prepare samples for testing.
-Demonstrate the ability to properly handle, transport, and store samples.
-Use statistical analysis, including the standard deviation, to determine the validity of data.
-Demonstrate accuracy and consistency in all laboratory procedures.
-Perform calibration of laboratory equipment as specified by laboratory protocols and/or manufacturer's specifications.
-Perform daily monitoring of laboratory equipment and keep quality assurance records of these values.
-Report all faulty/damaged equipment and equipment that is not operating within acceptable operational ranges (i.e., temperature, pressure, etc.)
-Assess the acceptability and appropriateness of a specimen submitted for laboratory testing.

Scientific Method

-Use the scientific method to conduct a valid experiment, including hypothesis formation, data collection, and data analysis.
-Develop scientific questions, hypotheses, and experimental plans.
-Evaluate the validity of results obtained during experimentation and product development.
-Evaluate scientific reports with well-supported, clearly presented opinions.

Cleanroom Protocols

-Describe the purpose of maintaining a cleanroom environment.
-Identify the potential sources of contamination that can affect a cleanroom environment.
-Describe appropriate dress and personal hygiene when working in a cleanroom environment.
-Demonstrate an understanding of correct gowning protocols.
-Describe the protocols for entering and exiting a cleanroom.
-Identify the concerns and appropriate protocols for transport of materials into and out of a cleanroom environment.
-Outline general housekeeping protocols for a cleanroom environment.

Water Purification Systems

-Distinguish between distilled and deionized water.
-List all the purification steps found in the facility's water purification system and describe briefly what each step accomplishes.
-Explain what is meant by type I water and why it cannot be stored for any length of time.
-Describe how the quality of type I water is monitored.
Lab Technician - CTTC
Perform Concentration of Solutions Calculations.
Career Majors That Sequence This Course
Career Cluster Pathway Career Major
Health Science Biotechnology Research and Development Assistant Laboratory Animal Science Technician
Health Science Biotechnology Research and Development Biological Laboratory Assistant
Health Science Biotechnology Research and Development Biological Laboratory Technologist I
Health Science Biotechnology Research and Development Biological Laboratory Technologist II
Health Science Biotechnology Research and Development Laboratory Animal Science Technician
Health Science Biotechnology Research and Development Laboratory Animal Science Technologist