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Course Information
Science/Technology/Engineering/Math
ST00018
Biotechnology II
120.00
This course is an advanced continuation of Biotechnology I. It will provide the student with practice in in vitro DNA synthesis reactions, programming and use of thermal cyclers for PCR reactions, utilization of real time PCR, Southern and Western blotting techniques, protein extraction and analysis, ELISA technology, and maintenance of animal cell lines.
Tina Fugate
405-743-5139
tfuga@okcareertech.org
or
Becki Foster
405-743-5554
bfost@okcareertech.org
Biology I
Chemistry
Biotechnology I




In vitro DNA Synthesis Reactions

Programming & Use of Thermal Cyclers for PCR reactions

Utilization of real time PCR

Southern & Western Blotting Techniques

Protein Extraction & Analysis

ELISA technology

Maintenance of animal cell lines

Advanced Bio-Informatics

Diagnostic Biotechnology: Forensics, RFLP DNA fingerprinting, disease diagnosis

Biofuels

Bioremediation

Pharmaceutical Production

Gene Therapy

Human Genome Project






Referenced Standards
National Science Standards (5th ed). (1998). National Research Council, Washington, D.C., National Academy of Sciences



Oklahoma Priority Academic Student Skills (2003). Oklahoma State Department of Education-PASS-{ www.sde.state.ok.us}
Biotechnology Industry Standards
Clark & Russell, (2000), Molecular Biology Made Simple and Fun, Cache River Press.

Barnum, Susan R., (2007), Biotechnology: An Introduction, Brooks/Cole.

Daugherty, Ellyn, (2007), Biotechnology: Science for the New Millennium, Paradigm Publishing.

Thieman and Palladino, (2004), Introduction to Biotechnology, Pearson Benjamin Cummings.

Micklos and Freyer, (2003), DNA Science: A First Course, Cold Spring Harbor Press.

Alcamo, (2001), DNA Technology: The Awesome Skill, Harcourt Academic Press.

Campbell and Reece, (2005), Biology: AP Edition, Pearson Benjamin Cummings.
No
8703
Yes
Yes
Yes
Career Majors That Sequence This Course
Career Cluster Pathway Career Major
Agriculture, Food & Natural Resources Plant and Soil Science Agri Technology
Science, Technology, Engineering & Mathematics Science and Mathematics Biotechnology